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KMID : 0616120100410030166
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Korean Journal of Pharmacognosy
2010 Volume.41 No. 3 p.166 ~ p.173
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Antioxidant Activity and Protective Effects of 9-hydroxy--tocopherone from Viola mandshurica Extracts
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Lee Mi-Ra
Hwang Ji-Hwan
Park Jae-Hee
Kim Hyun-Jung
Park Eun-Ju
Park Hae-Ryong
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Abstract
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Oxidative stress to proteins, lipids, or DNA is higher in human autopsy tissue and in rodent models of a number of neurodegenerative conditions, including Alzheimer’s and Parkinson’s disease. On the basis of this information, we established a screening system using N18-RE-105 cells to identify therapeutic agents that can protect cells from glutamate toxicity. During the course of our screening program, we recently isolated the active compound 9-hydroxy--tocopherone (-TP), which prevents glutamate-induced cell death, from Viola mandshurica. The chemical structure of -TP was identified using spectroscopic methods and by comparison with literature values. Antioxidant activity and protective effects of -TP were evaluated by DPPH radical-scavenging assay, morphological assay, MTT reduction assay, and lactate dehydrogenase (LDH) release assay. These results suggest that -TP could be a new potential chemotherapeutic agent against neuronal diseases.
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KEYWORD
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9-hydroxy--tocopherone, Viola mandshurica, glutamate, antioxidant activity, protective effect, N18-RE-105 cells
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